Search results for “PCR”

About 44 results in articles

Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing articles matching PCR — open any to read the full text, or download the PDF or XML.

Generation of a Single-Domain Antibody against Isolated Escherichia Coli that Causes Camel-Calf Death

Mar 2021 DOI 10.14302/issn.2575-1212.jvhc-21-3767
Faysal Abdishakur HassanCorresponding author

Antibodies and antibody fragments, especially single-domain antibodies known as nanobodies, are important tools in diagnostics, research, and therapeutics. In a conventional antibody, light and heavy chains contribute to the formation of the antigen binding site. In addition to conventional antibodi…

Clinical Evaluation of Significance of 25(Oh)D (Vitamin D) Status in Swine Flu (H1N1)

Sep 2020 DOI 10.14302/issn.2379-7835.ijn-20-3369
Bal Kishan GuptaCorresponding author

Background Since swine flu has been declared pandemic in 2009 it has become a major challenging public-health problem associated with high morbidity and mortality. 25(OH)D deficiency is also pandemic and has been reported to be clinically correlated with decreased immunity and respiratory infections…

Aerobic Plate Count of Contaminants and Molecular Characterization of Eschereichia Coli in Raw Chicken Meat in Ismailia, Egypt

Sep 2020 DOI 10.14302/issn.2575-1212.jvhc-20-3477
Fahim A ShaltoutCorresponding author

A total number of 100 samples from ten random broiler chicken carcasses (breast and thigh) were collected from an automatic poultry slaughtering plant in Ismailia city, Egypt. The mean values of Enterobacteriacae count were 5.9x104±9.7x103 cfu/g and 7.1x 104 ± 1.1x104 cfu/g for chicken breast and th…

Extraosseous Plasmacytoma of Thyroid Arising in Hashimoto’s Thyroiditis

Sep 2020 DOI 10.14302/issn.2372-6601.jhor-20-3552
Xin QingCorresponding author

Plasma cell neoplasms of the thyroid gland are uncommon. They may occur either as a primary extraosseous (extramedullary) plasmacytoma or as secondary involvement by multiple myeloma (MM). Here, we report the case of a 62-year-old female, presenting with goiter and Hashimoto’s thyroiditis, in whom t…

Molecular Analysis of 6-pyruvoyltetrahydropterin Synthase Gene in Atypical Phenylketonuric Egyptian Patients

Jul 2020 DOI 10.14302/issn.2576-6694.jbbs-20-3450
Magdy M. MahmoudCorresponding author

Background Hyperphenylalaninemia (HPA) combined with neurological signs due to impaired catecholamine, dopamine and serotonin synthesis. Symptoms may appears in first week of life but most seen in age of 4 months. Atypical PKU disease caused mainly by deficiency in 6-pyruvoyltetrahydropterin synthas…

Molecular Study of Hepcidin HAMP (-582A/G) Gene Polymorphisms and Measurement of Serum Hepcidin Level among Sudanese Patients with Anemia of Chronic Kidney Disease

May 2020 DOI 10.14302/issn.2575-7881.jdrr-20-3343
Amged Hussen AbdelrhmanCorresponding author

Background Anemia of chronic disease is anemia found in certain chronic disease states, is typically marked by the disturbance of iron homeostasis or hypoferremia. Chronic renal failure is currently known as Chronic Kidney Disease (CKD) or Chronic Renal Insufficiency (CRI) implies long-standing, pro…

Characterization of rpoB Gene Mutations Associated with Rifampicin Resistance in Multidrug Resistant Tuberculosis Patients Co-infected with HIV from Southern India.

Jul 2019
Shivaji K JadhavCorresponding author

Co-infection of HIV with Mycobacterium tuberculosis is a common event, particularly in developing countries. The emergence and spread of multidrug resistant tuberculosis (MDR-TB) is an increasing public problem in India. The drug-resistant M. tuberculosis strains are posing a significant challenge t…

Metal Metalloproteinase 2 is Differentially Regulated by the Interplay between Glucose and Insulin

Nov 2018
Ming-Yuh ShiauCorresponding author

Pre-adipocytes are the precursors with the potential to make new fat cells during adipose tissue expansion. Nevertheless, the pre-adipocytes behaviors, and their possible roles in energy homeostasis have long been overlooked. Our previous study implicates that interleukin-4 (IL-4) plays a positive m…

Is the Measles Virus Indeed Involved in Carcinogenesis? – Commentary

Aug 2018 DOI 10.14302/issn.2641-9181.ijnr-18-2195
Daniel BenharrochCorresponding author

Objectives: An association between the measles virus and Hodgkin lymphoma has been disclosed by our laboratory in Beer-Sheva, starting in 2003. We question the refutation of our study and the absence of interest among experts. Methodology: It was based on immunohistochemistry with commercial, as wel…

Genetic Diversity of Large Japanese Field Mouse Apodemus speciosus Populations and Identification of their Food Plant Resources using DNA Barcoding in an Industrial Green Space

May 2018 DOI 10.14302/issn.2637-6075.jpae-18-2062
Taichi FujiiCorresponding author

Estimates of the genetic diversity of Large Japanese field mouse Apodemusspeciosus populations and identification of their plant food resources were conducted in an industrial green space, where were constructed on reclaimed land and belonged to the Aichi Refinery of Idemitsu Kosan Co., Ltd., in Aic…

Journal of Enzymes Open Access

Using A “Superrooting”Cultivar of Taxus Chinensis Var. Mairei to Unravel Antioxidative Enzymes’ and Micrornas’ Role on Adventitious Rooting

May 2018 DOI 10.14302/issn.2690-4829.jen-18-2048
Wei TangCorresponding author

Rooting of cuttings is very important for production of economically important plants. We produced thousands of plantlets in Taxus chinensisvar. mairei using the technology of rooting of cuttings and identified two types of rooted cuttings, one with low rate of root formation and another with high r…

Hepatic Tuberculosis of Pseudotumor Form

Mar 2018 DOI 10.14302/issn.2578-2371.jslr-18-1994
Meriam SabbahCorresponding author Department of gastroenterology, Habib Thameur Hospital, Tunis, Tunisia.

Tuberculosis involving the liver in the absence of active pulmonary tuberculosis is very rare. The inflammatory pseudotumoral form is an entity difficult to diagnose. We report a case of an inflammatory pseudotumor of the liver due to tuberculosis, who didn’t underwent hepatectomy because of the siz…

Identification and Expression Analysis of Fragaria Vesca MLO Genes Involved in Interaction with Powdery Mildew (Podosphaera Aphanis)

Nov 2017 DOI 10.14302/issn.2638-4469.japb-17-1838
Shridhar JambagiCorresponding author

Strawberry powdery mildew, caused by Podosphaeraaphanis is a major fungal disease that affects strawberry yield and quality. In the model plant species Arabidopsis and the crop plants barley, tomato and pea, the Mildew resistance locus O (MLO) proteins have been found to be required for powdery mild…

Study of VCAM-1 Gene Expression in Normal and Tumoral Tissues in Patients with Colorectal Cancer

Nov 2017 DOI 10.14302/issn.2576-6694.jbbs-17-1744
Frouzandeh MahjoubiCorresponding author

Aim: Colorectal cancer is one of the most commonly diagnosed cancers in the world. Cell adhesion molecules play an important role in the progression of various cancers. It has been shown that the high level expression of some Cell adhesion molecule could be a new diagnostic factor for several cancer…

Signal Transduction of hCG Induces Decidualization and Uterine Receptivity

Aug 2017 DOI 10.14302/issn.2576-2818.jfb-14-553
L BernardiniCorresponding author

All independent experimental data on epithelial and glandular cells lines of human endometrium support the evidence for a rapid production of eicosanoids from the LH/hCG receptors when exposed to the hCG hormone. Prostaglandins rapidly act on the surrounding endometrial stromal cells throughout the…

Association of Epstein-Barr Virus with Gastric Carcinoma among Sudanese Patients

Oct 2016 DOI 10.14302/issn.2572-3030.jcgb-16-1190
Ibtihal omerCorresponding author

Background: Gastric cancer (GC) ranks as the fourth most common cancer and the second leading cause of cancer deaths worldwide. Epstein-Barr virus is a well-known oncogenic virus, it is responsible for 10% of gastric carcinomas across the world. The aim of study was to determine the prevalence of EB…

A study on the association of ACE i/D gene polymorphism, Obesity, Blood pressure and susceptibility of type 2 diabetes mellitus among the Kurmis of West Bengal, India.

Jun 2016
Arup Ratan BandyopadhyayCorresponding author

Earlier studies reported significant association of obesity, hypertension and Type2 Diabetes Mellitus (T2DM). Genetic and many disease-associated alleles have been identified through GWAS and applied to T2DM and indicated roles of renin-angiotensin system (RAS) in insulin signaling pathway and insul…

Ciliary Neurotrophic Factor Activated Signaling Pathways in Retinal Müller Cells

Mar 2016 DOI 10.14302/issn.2470-0436.jos-15-739
Vijay P. SarthyCorresponding author

Ciliary neurotrophic factor (CNTF) is a well-tested, neuroprotective agent that has been shown to retard photoreceptor degeneration in several animal models of retinitis pigmentosa. The molecular mechanisms underlying CNTF-mediated neuroprotection are currently not understood. CNTF could act directl…

The Role of BCL-2 and BAK Genes in Chronic Kidney Disease and Haemodialysis Patients

Feb 2016 DOI 10.14302/issn.2572-5424.jgm-15-815
Samah Abd ElhamidCorresponding author

Background: Polymorphonuclear leucocytes are the first line of defence against foreign invaders and constitute the major cell type involved in certain types of acute and chronic inflammatory diseases. Aim of the Work: The aim of the present study was to investigate the changes in expression of BCL-2…

Investigations of Molecular Evolutionary Mechanisms in Partially Sequenced Heat Shock Protein70 Homologue-Coding Gene of Olive Leaf Yellowing-Associated Virus Isolates from Tunisia

Feb 2016 DOI 10.14302/issn.2575-7881.jdrr-15-849
Moncef BOULILACorresponding author

Reverse Transcription Polymerase Chain Reaction (RT-PCR) using new designed primers pair for Heat Shock Protein70 homologue (HSP70h) of Olive leaf yellowing-associated virus revealed 667 amplified product of 10 olive accessions collected from various olive-growing regions in Tunisia. Amplicons were…

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