Search results for “assay”

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Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing articles matching assay — open any to read the full text, or download the PDF or XML.

Detection of Smuggled Genetically Modified Crops and Assessment of its Environmental Impact in the Ethio -Sudan Trans Boundary Area, North West Ethiopia

Apr 2023 DOI 10.14302/issn.2576-6694.jbbs-22-4390
Nega BerhaneCorresponding author

Background The genetic material of the genetically modified crop has been altered to develop the necessary insect resistance features by introducing genes from the Bt (Bacillus thuringiensis) bacterium. The objective of this study was to find smuggled GM Bt crops in the Metema farming area and exami…

Cytokine Expression in Peripheral Blood Mononuclear Cell Cultures Obtained from Cattle with Different Stages of Natural Mycobacterium bovis Infection

Dec 2021 DOI 10.14302/issn.2575-1212.jvhc-21-4034
Fernando Díaz-Otero,Corresponding author CENID-Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Carretera México-Toluca, Km. 15.5, C.P. 05110, Ciudad de México, México.

In bovine tuberculosis (bTB), cellular, humoral, or both types of immune responses have been observed. The purpose of this study was to examine the immune status of tuberculous cows based on the differential cytokine gene expression associated with Th1 (IFN-γ, IL-2), or Th2 (IL-4, IL-10) responses.…

Serum Ferritin Level as a Prognostic Marker of 30 days In Hospital Mortality of Coronavirus Disease 2019 (COVID 19) Pneumonia at World Citi Medical Center: A Retrospective, Observational Cohort, Single Center Study

Dec 2021 DOI 10.14302/issn.2692-1537.ijcv-21-4025
Allen M. QuiritCorresponding author

Introduction The COVID-19 pandemic continues to affect a large swath of the global population. The Philippine records four hundred seventy-four thousand sixty-four (474, 064) confirmed COVID 19 cases since December 31 2020. The COVID 19 pandemic recently highlighted the role of systemic hyperferrite…

Ameliorative Potential of Chlorogenic Acid on Rotenone-Induced Neurotoxicity in Drosophila Melanogaster Model

Nov 2021 DOI 10.14302/issn.2641-4538.jphi-21-3993
Oluwatoyin Adenike Adeyemo-SalamiCorresponding author

Chlorogenic acid (CA), abundantly found in green coffee beans, is a phenolic compound with antioxidant and anti-inflammatory properties amongst others. Exposure to rotenone, a natural pesticide, induces Parkinsonism (a type of neurodegeneration) through the induction of mitochondria dysfunction and…

SARS-Cov-2 Viral Kinetics in Mild COVID-19 Patients Treated with Chloroquine Regimens or Standard of Care

Aug 2021 DOI 10.14302/issn.2692-1537.ijcv-21-3924
Krittaecho SiripassornCorresponding author

This study measures the impact of chloroquine (CQ) therapy in reducing SARS-CoV-2 viral load in infected individuals and hence its transmissibility by describing changes in nasopharyngeal SARS-CoV-2 RNA kinetics in patients receiving standard of care (SOC) or CQ +/- ritonavir-boosted lopinavir (LPV/…

Evaluation of Renal and Cardioprotective Potential of the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats

Jun 2021 DOI 10.14302/issn.2574-4488.jna-21-3847
Snehasis JanaCorresponding author

The aim of this study was to evaluate the impact of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on kidney biomarkers on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats. In this experiment, the funct…

Effect of Wheat Germ, Wheat Germ Oil on Lipid Profile of Hypercholesterolemic Rats

Dec 2020 DOI 10.14302/issn.2835-513X.ijl-20-3247
Ghada Z A SolimanCorresponding author

Hypercholesterolemia plays an important role in atherosclerosis and cardiovascular diseases (CVD) that represent one of the greatest worldwide medical problems nowadays. Recently, an increased attention/ interest for natural antioxidant/ hypercholesterolemia as WG/WGO is increasing. Wheat germ/ Whea…

Clinical Evaluation of Significance of 25(Oh)D (Vitamin D) Status in Swine Flu (H1N1)

Sep 2020 DOI 10.14302/issn.2379-7835.ijn-20-3369
Bal Kishan GuptaCorresponding author

Background Since swine flu has been declared pandemic in 2009 it has become a major challenging public-health problem associated with high morbidity and mortality. 25(OH)D deficiency is also pandemic and has been reported to be clinically correlated with decreased immunity and respiratory infections…

Effects of Selected Secondary Metabolites in Leaf Extract of Jatropha Tanjorensis on Some Gonadal Hormones in Male Wistar Rats

Jul 2020 DOI 10.14302/issn.2576-6694.jbbs-20-3466
John AkighirCorresponding author Department of Biochemistry, College of Science, Federal University of Agriculture, Makurdi P.M.B. 2373 (970001) Nigeria

Background and Objective The use of medicinal plants in industrialized societies for extraction and development of many drugs and other chemotherapeutics and traditionally for herbal remedies has increased in recent times. Plant–based medicine is essential in health care services with about 80% glob…

Efficacy of Phytochemical Constituents of Castor Essential oil Towards the Mucor-Mycotic Mold Cunninghamella Bertholletiae

Jul 2020 DOI 10.14302/issn.2377-2549.jndc-20-3484
S.M. Shakeel IqubalCorresponding author

The aim of this experiment is to study the efficacy of phytochemical constituents of Castor essential oil towards the mucor-mycotic mold Cunninghamella bertholletiae.The standard chemical analytical methods were used for the rapid study of the phytochemical constituents responsible for the antimicro…

Antioxidant Activity of Surinamese Medicinal Plants with Adaptogenic Properties and Correlation with Total Phenolic Contents

Jul 2020 DOI 10.14302/issn.2471-2140.jaa-20-3478
Dennis R.A. MansCorresponding author

Plant-based preparations are commonly used in Suriname (South America) as adaptogens. In this study, fifteen alleged adaptogenic Surinamese plants have been assessed for their antioxidant activity (AA), total phenolic contents (TPC), and total flavonoid contents (TFC). The investigated plants were A…

Molecular Study of Hepcidin HAMP (-582A/G) Gene Polymorphisms and Measurement of Serum Hepcidin Level among Sudanese Patients with Anemia of Chronic Kidney Disease

May 2020 DOI 10.14302/issn.2575-7881.jdrr-20-3343
Amged Hussen AbdelrhmanCorresponding author

Background Anemia of chronic disease is anemia found in certain chronic disease states, is typically marked by the disturbance of iron homeostasis or hypoferremia. Chronic renal failure is currently known as Chronic Kidney Disease (CKD) or Chronic Renal Insufficiency (CRI) implies long-standing, pro…

Effect of Hexavalent Chromium on the WBCs of the Fresh Water Fish, Labeo rohita

May 2020 DOI 10.14302/issn.2641-7669.ject-20-3313
A.J ThatheyusCorresponding author PG & Research Department of Zoology, The American College, Madurai, Tamil Nadu, India.

Industrial effluents containing heavy metals may reach aquatic systems either through direct discharge or surface runoff and cause damage to aquatic organisms affecting their immune system and health. Hence the present study has been undertaken to observe the effects of hexavalent chromium on the WB…

Invivo Impact of Malaria and HIV Co-Infection on CD4 Cell Count of Infected Patients of Niger Delta Extraction

May 2020 DOI 10.14302/issn.2328-0182.japst-20-3347
Obioma AzuonwuCorresponding author Department of Medical Laboratory Science, Medical Bacteriology / Virology / Parasitology Unit, Rivers State University, Nkpolu – Oroworukwo, Port Harcourt, Rivers State, Nigeria.

The study evaluated the impact of co-infection of malaria parasitaemia, and HIV positive indices on the CD4 cell count of 120 HIV infected subjects, who were already diagnosed and visiting Braithwaite Memorial Specialist Hospital Port Harcourt for routine Medical check-up. Also, a control group of 4…

RETRACTED: Assessment of the Risk of Hemochromatosis in Polytransfused Sickle Cell Patients at the Abidjan Transfusion Therapy Unit

Apr 2020 DOI 10.14302/issn.2372-6601.jhor-20-3189
Mamadou Sekongo YassonguiCorresponding author Department of Training and Research, National Blood Transfusion Center; Abidjan; Côte D’Ivoire

This article has been retracted on 29 January 2021. VIEW THE RETRACTION NOTICE (https://doi.org/10.14302/issn.2372-6601.jhor-25-5854) In Côte d'Ivoire, sickle cell disease affects 14% of the population. It is responsible for significant morbidity and mortality. Transfusion is a significant element i…

Auricularia Polytricha (Mushroom) Regulates Testicular DNA Expression and Oxidative Stress Markers of Streptozotocin-Induced Diabetic Male Wistar Rat

Mar 2020 DOI 10.14302/issn.2379-7835.ijn-20-3175
Cyril Abang AgborCorresponding author Department of Anatomy, Collage of Basic Medical Sciences, University of Calabar, Nigeria

Local Nigerian men have been using AuriculariaPolytricha as a treatment for sexual dysfunction without supporting evidence from scientific experiments. This study was to investigate the effect of ethanolic extract of A. Polytricha on testicular DNA expression and some oxidative stress markers using…

The Effect of 2,4 Dimethylamine salt on the Blood, Liver and Muscle of Oryclotagus Cuniculus

Mar 2020 DOI 10.14302/issn.2637-6075.jpae-20-3198
Sylvester C. IzahCorresponding author

Aminoforce containing 720g/l of 2,4-dimethylamine salt induced changes on some enzymes and electrolytes in the male Oryclotagus cuniculus (New Zealand rabbit) were assayed. The organisms were exposed to varying sub-lethal concentrations of the toxicant (720g/l). The concentrations were prepared by p…

Interest of Confirmation Tests in the Diagnosis of Viral Hepatitis C to Blood Donors in Abidjan-Côte d'Ivoire

Jan 2020 DOI 10.14302/issn.2372-6601.jhor-20-3186
Yassongui Mamadou SekongoCorresponding author

Introduction The anti-HCV RIBA test verifies the presence of anti-HCV serum antibodies detected by the Elisa test. In Côte d'Ivoire, screening for hepatitis C is done exclusively by enzyme immunoassays. In order to reduce the number of HCV positive blood donor exclusions on ELISA, we conducted this…

Effect of the Biofield Energy Treated Proprietary Test Formulation for Sleep Biomarkers in the Unpredictable Chronic Stress (UCS) Animal Model 

Jan 2020 DOI 10.14302/issn.2474-9273.jbtm-19-3157
Snehasis JanaCorresponding author

Sleep biomarkers in brain such as melatonin, BDNF (Brain-derived neurotrophic factor), PGD2 (Prostaglandin D2), leptin, orexin-A, and acetylcholine were evaluated in the unpredictable chronic stress (UCS) rodent model in the presence of Consciousness Energy Healing Treated (the Trivedi Effect®) nove…

In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (Portulacaceae)

Jan 2020 DOI 10.14302/issn.2379-7835.ijn-19-3144
Yaaser Q. AlmulaikyCorresponding author

In this study, the antioxidants and photosynthetic compounds of Verdolaga were examined. Compounds were extracted from distinctive segments of the verdolaga using various solvents such as methanol (40, 60, 80%), ethanol (40, 60, 80%), acetone (40, 60, 80%), and deionized water. The use of 80% methan…

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